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96 Wells Porcine Matrix Metalloproteinase 3 ELISA Kit High Sensitivity

Categories Porcine ELISA Kit
Brand Name: BT Lab
Model Number: Cat.No E0455Po
Certification: CE, ISO9001:2005, MSDS
Place of Origin: Shanghai, China
MOQ: 1 kit
Price: Negotiation
Payment Terms: Western Union, T/T
Supply Ability: In Stock
Delivery Time: 1-3 business days, bulk order within one week
Packaging Details: Wrapped with ice pack and styrofoam package
Target Protein: Matrix Metalloproteinase 3
Sample: serum,plasma,urine,tissue,cell culture supernatant
Assay Time: 2 hours
Discount: Available
Bulk Order: Yes
Assay Principle: Sandwich
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96 Wells Porcine Matrix Metalloproteinase 3 ELISA Kit High Sensitivity

Specificity Porcine Matrix Metalloproteinase 3 ELISA Kit 96 wells


Cat.No E0455Po


Reagent Provided

ComponentsQuantity
Standard Solution (96ng/ml)0.5ml x1
Pre-coated ELISA Plate12 * 8 well strips x1
Standard Diluent3ml x1
Streptavidin-HRP6ml x1
Stop Solution6ml x1
Substrate Solution A6ml x1
Substrate Solution B6ml x1
Wash Buffer Concentrate (30x)20ml x1
Biotinylated Porcine MMP-3 Antibody1ml x1
User Instruction1
Plate Sealer2 pics
Zipper bag1 pic

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (96ng/ml) with 120μl of standard diluent to generate a 48ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (48ng/ml) 1:2 with standard diluent to produce 24ng/ml, 12ng/ml, 6ng/ml and 3ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:


48ng/mlStandard No.5120μl Original Standard + 120μl Standard Diluent
24ng/mlStandard No.4120μl Standard No.5 + 120μl Standard Diluent
12ng/mlStandard No.3120μl Standard No.4 + 120μl Standard Diluent
6ng/mlStandard No.2120μl Standard No.3 + 120μl Standard Diluent
3ng/mlStandard No.1120μl Standard No.2 + 120μl Standard Diluent

Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
96ng/ml48ng/ml24ng/ml12ng/ml6ng/ml3ng/ml

Product Tags:

elisa reagent kit

  

elisa test kit

  
China 96 Wells Porcine Matrix Metalloproteinase 3 ELISA Kit High Sensitivity factory
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